New technique for mouse oocyte injection via a modified holding pipette
نویسندگان
چکیده
منابع مشابه
INTRACYTOPLASMIC SPERM INJECTION (lCSI) IN MICE BY A NEW HOLDING PIPETTE
Mice are ideal models for ICSI experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. Unfortunately, it has been extremely difficult. In this study we improved the mouse ICSI method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. The outer pipette ...
متن کاملintracytoplasmic sperm injection (lcsi) in mice by a new holding pipette
mice are ideal models for icsi experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. unfortunately, it has been extremely difficult. in this study we improved the mouse icsi method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. the outer pipette had ...
متن کاملProduction of Sry knockout mouse using TALEN via oocyte injection
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the Y chromosome, using microinjection of TALEN RNA into pronuclear stage oocytes. As expected, the knockout mouse had female external and internal genit...
متن کاملComplete oocyte activation failure after ICSI can be overcome by a modified injection technique.
BACKGROUND Complete fertilization failure after ICSI is a rare event, and it may happen repeatedly even in cases of normal sperm parameters and good ovarian response. In these cycles, alternative ICSI techniques may prove useful. METHODS Our modified ICSI (mICSI) is characterized by aspiration close to the opposite membrane (the region of the mitochondria with a high inner mitochondrial membr...
متن کاملOocyte Enucleation Phase in Mouse Cloning
Mouse oocytes were placed in Hepes-T6 containing 7.5 μg/ml cytochalasin B and 5μg/ml Hoechst prior to enucleation, and enucleation was performed on oocytes using the manipulator. The oocyte held in place by gentle suction of a holding pipette (oocyte diameter approx.70 μm). The metaphase spindle (arrow) is aspirated into an enucleation pipette (1).
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2010
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2010.07.004